Ol Pajeta Conservancy on Tuesday, March 20 said they chose to euthanise him after the condition deteriorated extensively.
The 45-year-old rhino was euthanized Monday after his conditions "worsened significantly" and he was unable to stand any longer, the Ol Pejeta Conservancy in Kenya said in a statement.
The conservancy continues to take care of two female northern white rhinos - Najin and Fatu - who are also suffering from various health complications and were infertile.
Sudan's death "is a cruel symbol of human disregard for nature and it saddened everyone who knew him".
He had been born in Sudan, taken to a Czech zoo and then transferred to Kenya in 2009.
Sudan even joined Tinder previous year, as the zoo tried to raise awareness about his threatened species and raise money for reproductive research.
Fatu and Najin are now the last rhinos of this kind in the world and the fate of the subspecies lies in their ability to become pregnant through IVF.
World's last male northern white rhino Sudan dies of 'age-related complications'
Sudan's genetic material was collected on Monday for future attempts at reproduction of northern white rhinos through advanced cellular technologies.
However, the conservancy vet Stephen Ngulu said they had managed to bring the situation under control using painkillers and antibiotics, and Sudan had regained his appetite.
More: World's last male northern white rhino is gravely ill.
Elodie Sampere, a representative for Ol Pejeta, said: "He was a gentle giant, his personality was just unbelievable and given his size, a lot of people were afraid of him".
However, demand for rhino horn in traditional Chinese medicine and dagger handles in Yemen fueled a poaching crisis in the 1970s and 1980s that largely wiped out the northern white rhino population in Uganda, Central African Republic, Sudan and Chad.
The rhino "significantly contributed to survival of his species as he sired two females", the conservancy said.
Sudan gained worldwide fame in 2017 after he was featured on the popular dating app Tinder in an effort to raise money for the IVF procedure - which has never been done with rhinos.
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