As a freakish quirk, Hugo was wearing the No.31 shirt when he scored exactly 13 minutes after the Astori tribute - at 13:00pm on the dot - on his 13th appearance for La Viola.
Thousands of supporters turned out to pay their respects at the defender's funeral in Florence on Thursday, and fans continued their tributes on Sunday as Fiorentina returned to domestic action against Benevento at Stadio Artemio Franchi.
Astori, who made 289 top-flight appearances, joined Fiorentina on loan in August 2015 and the club signed him outright one year later. Astori's number thirteen shirt has been retired by the club in his honour.
Does recently declassified Navy video show a UFO?
Chris Mellon, an adviser to TTSA and a former deputy assistant secretary of defense for intelligence for the Clinton and George W. The 35 second clip was reportedly captured by an infrared camera aboard a F/A-18 fighter jet travelling at 25,000 feet in 2015.
Vitor Hugo scored the winning goal in the first half as La Viola remembered Astori before, after and during the match.
Both teams hit the post in the final minutes, through Massimo Coda and new Fiorentina captain Milan Badelj.
In the end, however, Hugo's header proved to be the difference between the two sides on a day when football seemed to matter little.